Revisiting the Closed-Loop Model and the Nature of mRNA 5'-3 ...
WEBDec 6, 2018 · Here, we address fundamental questions about the closed-loop model and discuss how a growing understanding of mRNA structure, dynamics, and intermolecular interactions presents new experimental opportunities.
Revisiting the closed loop model and the nature of mRNA 5′–3 ...
WEBDec 6, 2018 · Here, we briefly summarize evidence for, and issues with, the generally accepted version of the mRNA closed loop model. We discuss some new ways we might think about the nature of 5′–3′ proximity and propose possible avenues for exploration.
Roles of mRNA poly (A) tails in regulation of eukaryotic gene ...
WEBSep 30, 2021 · The closed-loop model for how PABPC controls mRNA metabolism is enticing and has made its way into numerous textbooks as canon.
Single-molecule visualization of mRNA circularization during ...
WEBJan 31, 2023 · During the process, mRNA undergoes functional circularization (or becomes a “closed loop”) through protein–mRNA and protein–protein interactions to positively regulate protein synthesis 3...
Making ends meet: New functions of mRNA secondary structure
WEB3.3 ∣. mRNA compactness and the closed-loop model of translation initiation. Another example of protein-mediated interactions between the 5′ cap and 3′ poly(A) tail is the formation of a closed-loop structure during translation initiation.
Probing the closed-loop model of mRNA translation in living cells
WEBThe mRNA closed-loop, formed through interactions between the cap structure, poly (A) tail, eIF4E, eIF4G and PAB, features centrally in models of eukaryotic translation initiation, although direct support for its existence in vivo is not well established.
Translation factors promote the formation of two states of the closed …
WEBMay 21, 2008 · The 5′ and 3′ ends of eukaryotic mRNAs are brought together to form a closed loop, which helps stabilize the mRNA and facilitates translation.
Probing Structural Dynamics of the Closed-Loop Model in …
WEBFeb 12, 2021 · To address this problem, we have developed single-molecule fluorescence assays to visualize when closed-loop factors are bound to the mRNA, while simultaneously probing mRNA end-to-end proximity (when mRNA ends are …
Revisiting the Closed-Loop Model and the Nature of mRNA 5′–3 ...
WEBDec 6, 2018 · Here, we briefly summarize evidence for, and issues with, the generally accepted version of the mRNA closed-loop model. We discuss some new ways we might think about the nature of 5′–3′ proximity and propose possible avenues for exploration.
mRNA length-sensing in eukaryotic translation: reconsidering …
WEBWe recently reported that the ribosomal signaling scaffold protein RACK1 promotes the efficient translation of short mRNAs that strongly associate with the closed loop factors.