After an additional washing step (PBS, pH 7.4, 20°C), cells were resuspended in 1 mL RPMI medium (20°C, 5.5 g/L NaCl, 5 mg/L phenol red, 2.0 g/L NaHCO 3, 25 mM HEPES ... and 2 mM EDTA), cells ...
Host cells activate cell death pathways in response to bacterial manipulation of innate immune signalling, but the molecular ...